Norambuena´s Reseach interest

Protein Trafficking: Regulation and Role in Plant Development

Plants, as sessile organisms, must adapt to changing environmental conditions. Plants are able of sensing and respond to both rapid and longer-term changes in their environment. As part of the adaptation process plants are capable of generate post-embryonic organs such as lateral roots and root hairs. The development of these structures strongly relies on the correct location and organization of the components of the cell wich is mainly performed by the endomembrane system. The endomembrane system is responsible for the destination and retention of proteins in the cell wall and its component compartments. It is also responsible for the maintenance of the cell structure as well as cell division and cell elongation. Endomembrane system plasticity allows to adapt and respond to cellular requirements and therefore when the entire organism faces environmental challenges. All these process are integrated and highly regulated. The interest of our laboratory is focused on understanding the mechanisms for the regulation of endomembrane system and its effect on post-embryonic development in plants.

Agro-chemical Biothecnology

Endomembrane system modulation have a strong impact on plant development as well in response to challenged enviromental conditions. In this context finding tools to modulate endomembrane system could be powerful to modulate plant response and adaptation mechanisms. In this context we are perfoming research on Agrochemical biothecnology. Taking advantages of chemical genomics approach we are seeking for chemical coumpounds that modulate endomembrane system as well as post-embryonic development with the aim of finding tools to improve plant performance.

Biological stratagies and aproaches

In our laboratory, we use : 1. Genome-wide broad search (“genome-wide screen”) of mutant organisms that have alterations in the plant endomembrane system. 2. Chemical genomics (“chemical genomics” or “chemical genetics” ), based on the use of chemical compounds able to alter, modify or inhibit the endomembrane system functions, which effect is dose dependent and reversible controlling the extent of the interest phenotype and manipulate their action temporarily. 3. Methodologies and tools for visualizing cellular protein trafficking. 4. Functional genomics to characterize gene and protein function 5. Tranciptomic and system biology approaches that help us to answer our biological interests.